- Clb5 Clb6 Mutants
- clb5∆ clb6∆
- TAB6-1 clb5∆ clb6∆
- cdc20∆ clb5∆
- cdc20∆ pds1∆ clb5∆
- CLB2-db∆ clb5∆
- CLB2-db∆ clb5∆ in galactose
- cln1∆ cln2∆ clb5∆ clb6∆
- CLB5-db∆
- CLB5-db∆ sic1∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- GAL-CLB5
- GAL-CLB5 cdh1∆
- GAL-CLB5 sic1∆
- GAL-CLB5-db∆
- cln1∆ cln2∆ cln3∆ multi-copy CLB5
- cln1∆ cln2∆ cln3∆ GAL-CLB5
GAL-CLB5-db∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksb5'=0.012, kdb5"=0, MDT=150.
Experiments:
Schwob, E., Bohm, T., Mendenhall, M.D. and Nasmyth, K. (1994). The B-type cyclin kinase inhibitor p40sic1 controls the G1 to S transition in S. cerevisiae. Cell 79:233-244.
[Abstract] [Article]
[Abstract] [Article]
Jacobson, M.D., Gray, S., Yuste-Rojas, M. and Cross, F.R. (2000). Testing cyclin specificity in the exit from mitosis. Mol. Cell. Biol. 20:4483-4493.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Lethal. Schwob, Fig. 7, DNA synthesis is not significantly advanced. Jacobson, Table 1 and Fig. 8, cells lose viability quickly upon galactose induction. Many cells are multiply budded. DNA contents ranges from unreplicated to apparent fully replicated. Loss of viability is attributable to the ability of these cells to divide but they do not efficiently replicate their DNA after division, accounting for the accumulation of cells with 1C DNA.
Comments: In simulation, this mutant exits the first mitosis but dies afterwards because of DNA replication problems. [ORI] (grey line) is not re-licensed after the first mitosis.
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